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Image Search Results
Journal: Veterinary World
Article Title: Molecular epidemiology of Megalocytivirus in freshwater angelfish ( Pterophyllum scalare ) from Johor, Malaysia
doi: 10.14202/vetworld.2023.2158-2172
Figure Lengend Snippet: The total number of positive bands obtained from initial environmental and current sampling.
Article Snippet: A total of 21 μL
Techniques: Sampling, Nested PCR
Journal: Veterinary World
Article Title: Molecular epidemiology of Megalocytivirus in freshwater angelfish ( Pterophyllum scalare ) from Johor, Malaysia
doi: 10.14202/vetworld.2023.2158-2172
Figure Lengend Snippet: The phylogenetic tree was constructed for 167bp segments based on MCP gene sequences of ISKNV detected in 10 of Megalocytivirus-positive samples from this study (black bold) and 6 reference viruses from the genus Megalocytivirus using the neighbour-joining method and 1000 bootstrap replicates. JQ253365.1 Megalocytivirus Sabah/RAA/2012 strain GGIV3 major capsid protein gene. FLIV-PH MCP gene (AY633992.1), FLIV-JSY MCP gene (AY633989.1) FLIV-DS1 MCP gene (AY633980.1), ISKNV MCP gene for major capsid protein, strain: DGA10/8 (AB666346.1), Sea bass iridovirus-K MCP gene for major capsid protein (AB178942.1), SFIV-P major capsid protein gene (KF499523.1), DGIV major capsid protein gene (AY989901.1), OFLIV-1 major capsid protein gene (EU276417.1), GSIV-K1 major capsid protein (MCP) gene (EU315313.1), Threespine stickleback iridovirus major capsid protein gene (HQ857785.1), Turbot iridovirus MCP gene for major capsid protein (AB166788.1), Sea perch iridovirus isolate CH-1 major capsid protein (MCP) gene (HM067603.1), ISKNV MCP gene for major capsid protein, strain: mullet iridovirus MA5/5 (AB666343.1), ISKNV MCP gene for major capsid protein, strain: DGIV DG12/8 (AB666348.1), ALIV gene for major capsid protein (AB109368.1), DGIV gene for major capsid protein (AB109369.1), DGIV major capsid protein gene (AY285744.1), Sea bass iridovirus major capsid protein gene (AY310917.1) and Spotted knifejaw iridovirus isolate SKIV-ZJ07 major capsid protein gene (GQ202216.1) were included as sister group. Tiger frog virus (AF 389451.1) and Frog virus 3 (AY 548484.1) (red bold) were included as outgroup. The scale bar indicated distance values, the GenBank accession numbers were presented next to the virus species/strains, and the bootstrap values (%) were demonstrated next to the clades. The tree is drawn to scale (0.050), with branch lengths measured in the number of substitutions per site.
Article Snippet: A total of 21 μL
Techniques: Construct, Virus
Journal: Veterinary World
Article Title: Molecular epidemiology of Megalocytivirus in freshwater angelfish ( Pterophyllum scalare ) from Johor, Malaysia
doi: 10.14202/vetworld.2023.2158-2172
Figure Lengend Snippet: Assessment results of association between the presence of Megalocytivirus in fish and water samples with regards to water parameters and fish age based on Pearson’s correlation coefficient analysis.
Article Snippet: A total of 21 μL
Techniques: Infection, Concentration Assay